Collagen processing, crosslinking, and fibril bundle assembly in matrix produced by fibroblasts in long-term cultures supplemented with ascorbic acid.

@article{Grinnell1989CollagenPC,
  title={Collagen processing, crosslinking, and fibril bundle assembly in matrix produced by fibroblasts in long-term cultures supplemented with ascorbic acid.},
  author={Frederick Grinnell and H Fukamizu and Peter D. Pawelek and Satoshi Nakagawa},
  journal={Experimental cell research},
  year={1989},
  volume={181 2},
  pages={483-91}
}
Human foreskin fibroblasts were cultured for up to 6 weeks in medium supplemented with ascorbic acid. During this time, the cells produced an extensive new connective tissue matrix in which the accumulated collagen (mostly type I) amounted to about 0.25 mg/10(6) cells. The matrix was highly differentiated as shown by complete processing of procollagen to collagen alpha-chains and covalent crosslinking of the collagen. Alignment of collagen fibrils occurred as the fibrils were deposited between… CONTINUE READING

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