Collaborative dynamic DNA scanning by nucleotide excision repair proteins investigated by single- molecule imaging of quantum-dot-labeled proteins.

@article{Kad2010CollaborativeDD,
  title={Collaborative dynamic DNA scanning by nucleotide excision repair proteins investigated by single- molecule imaging of quantum-dot-labeled proteins.},
  author={Neil M. Kad and Hong Wang and Guy G. Kennedy and David M. Warshaw and Bennett Van Houten},
  journal={Molecular cell},
  year={2010},
  volume={37 5},
  pages={702-13}
}
How DNA repair proteins sort through a genome for damage is one of the fundamental unanswered questions in this field. To address this problem, we uniquely labeled bacterial UvrA and UvrB with differently colored quantum dots and visualized how they interacted with DNA individually or together using oblique-angle fluorescence microscopy. UvrA was observed to utilize a three-dimensional search mechanism, binding transiently to the DNA for short periods (7 s). UvrA also was observed jumping from… CONTINUE READING

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