Colicin A unfolds during its translocation in Escherichia coli cells and spans the whole cell envelope when its pore has formed.

@article{Bndetti1992ColicinAU,
  title={Colicin A unfolds during its translocation in Escherichia coli cells and spans the whole cell envelope when its pore has formed.},
  author={H{\'e}l{\`e}ne B{\'e}n{\'e}detti and Roland Lloubes and Claude Lazdunski and Lucienne Letellier},
  journal={The EMBO journal},
  year={1992},
  volume={11 2},
  pages={441-7}
}
The addition of the pore forming colicin A to Escherichia coli cells results in an efflux of cytoplasmic potassium. This efflux is preceded by a lag time which is related to the time needed for the translocation of the toxin through the envelope. Denaturing the colicin A with urea, before adding it to the cells, did not affect the properties of the pore but decreased the lag time. After renaturation, the lag time was similar to that of the native colicin. This suggests that the unfolding of… CONTINUE READING

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