Cold storage of biopsies from wild endangered native Chilean species in field conditions and subsequent isolation of primary culture cell lines

@article{Tovar2008ColdSO,
  title={Cold storage of biopsies from wild endangered native Chilean species in field conditions and subsequent isolation of primary culture cell lines},
  author={Heribelt Tovar and Felipe I. Navarrete and Lleretny Rodr{\'i}guez and Oscar Skewes and Fidel Ovidio Castro},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  year={2008},
  volume={44},
  pages={309-320}
}
We evaluated the possibility of deriving primary cell cultures from tissue biopsies taken in field conditions from six threaten endemic Chilean species of free-ranging mammals. Biopsies were taken either by ear punching or darts fired to animals and hold in hypothermic conditions (4° C) in defined salt solution for time periods ranging from 0 to 7 d before… CONTINUE READING