Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.

@article{Kermekchiev2003ColdsensitiveMO,
  title={Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.},
  author={Milko B. Kermekchiev and Anatoly Tzekov and W. M. Barnes},
  journal={Nucleic acids research},
  year={2003},
  volume={31 21},
  pages={6139-47}
}
Although the thermophilic bacterium Thermus aquaticus grows optimally at 70 degrees C and cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20-37 degrees C. This activity is a bane to some PCRs, since it catalyzes non-specific priming. We report mutations of Klentaq (an N-terminal deletion variant) DNA polymerase that have markedly reduced activity at 37 degrees C yet retain apparently normal activity at 68 degrees C and resistance at 95 degrees C. The first… CONTINUE READING