Cold-sensitive Ca2+ Influx in Paramecium

The concentration of intracellular calcium, [Ca2+] i , in Paramecium was imaged during cold-sensitive response by monitoring fluorescence of two calcium-sensitive dyes, Fluo-3 and Fura-Red. Cooling of a deciliated Paramecium caused a transient increase in [Ca2+] i at the anterior region of the cell. Increase in [Ca2+] i was not observed at any region in Ca2… CONTINUE READING