Cold-plaque screening: a simple technique for the isolation of low abundance, differentially expressed transcripts from conventional cDNA libraries

@inproceedings{Hodge1992ColdplaqueSA,
  title={Cold-plaque screening: a simple technique for the isolation of low abundance, differentially expressed transcripts from conventional cDNA libraries},
  author={Rachel Hodge and Wyatt Paul and John Draper and Rod J. Scott},
  year={1992}
}
  • Rachel Hodge, Wyatt Paul, +1 author Rod J. Scott
  • Published 1992
  • Biology
  • Summary We report here a simple method for obtaining organ-or tissue-specific clones from a cDNA library which are represented at low or medium abundance in the mRNA pool. An anther cDNA library has been screened for anther-specific clones using a new technique we have called cold-plaque screening. Using cold-plaque screening we have confirmed the prediction that a large proportion (40%) of unique transcripts in the anther are organ-specific but that the majority are of low abundance and… CONTINUE READING

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