Cold-induced apoptosis of rat liver endothelial cells: involvement of the proteasome.

@article{Doeppner2003ColdinducedAO,
  title={Cold-induced apoptosis of rat liver endothelial cells: involvement of the proteasome.},
  author={Thorsten R Doeppner and T. Grune and Herbert de Groot and Ursula Rauen},
  journal={Transplantation},
  year={2003},
  volume={75 12},
  pages={1946-53}
}
BACKGROUND We have previously shown that in cultured liver endothelial cells the mere sequence of hypothermia and rewarming induces a pronounced, iron-dependent apoptosis that is likely to contribute to hepatic preservation injury. Here we study the involvement of proteases in this cold-induced apoptosis. METHODS Cultured liver endothelial cells were incubated in preservation solutions at 4 degrees C in the absence or presence of protease inhibitors. Cell injury and different protease… CONTINUE READING