• Corpus ID: 247411396

Cold atmospheric plasma activated deionized water using helium, argon, and nitrogen as feeding gas for cancer therapy

@inproceedings{Chen2022ColdAP,
  title={Cold atmospheric plasma activated deionized water using helium, argon, and nitrogen as feeding gas for cancer therapy},
  author={Zhitong Chen},
  year={2022}
}

• Zhitong Chen
• Published 6 March 2022
• Biology, Medicine

Plasma activated medium, containing reactive oxygen species (ROS) and reactive nitrogen species (RNS), has been used for cancer treatment as an indirect treatment method. Here we report cold atmospheric plasma (CAP) activated deionized (DI) water using helium (He), argon (Ar), and nitrogen (N2) as feeding gas for the cancer therapy. Basic characteristics of CAP activated DI water with different feeding gases were diagnosed and the effects of three solutions on the breast cancer cells and gastric cancer cells were also recorded. Our findings show that DI water treated by CAP with He feeding gas has a much stronger effect on apoptosis in precultured breast cancer cells and gastric cancer cells. These results are attributed to the higher concentration of ROS generated in CAP-activated DI water using He as feeding gas. CAP has been utilized in various traditional fields and is being gradually developed into more fields due to its unique nature. It’s known for the generation of reactive species (ROS and RNS), charged particles, electronically excited atoms, etc. Plasma containing plenty of composition leads to itself a broad range of applications such as environment, materials, agriculture, semiconductor, medicine, and aerospace. Among all these fields, plasma medicine is an innovative and emerging interdisciplinary research field that combines plasma physics, chemistry, life science, and clinical medicine. As the temperature of CAP is close or even lower than room 2 temperature, CAP seldom causes thermal damage to the human body and biological tissues. Due to the benefits from above, CAP is attracting increasing attention and has been used in sterilization, dental treatment, cosmetology, hemostasis, anti-inflammation, wound healing, and skin disease treatment. CAP has shown successful applications in cancer therapy both in vivo and in vitro, including oral cancer, lung carcinoma, throat cancer, breast cancer, neuroblastoma, hepatocellular carcinoma, melanoma, skin carcinoma, pancreatic carcinoma, colon carcinoma, cervical carcinoma, and bladder carcinoma. Fridman et al reported the anti-cancer effect of CAP in 2007, promoting plasma medicine to a new level. CAP tends to inhibit the growth of cancer cells rather than homologous normal cells in dozens of cell lines. CAP treatment can be directly applied to the tumor/tissues/cells. For example, Li et al employed the surface dielectric barrier discharge (SDBD) to treat the human hepatocellular carcinoma cells. In order to expand the application of CAP, plasma can be delivered through a tiny tube for cancer therapy due to the inaccessibility of conventional CAP devices. Chen et al. delivered CAP through micro-sized tube to glioblastoma, which effectively prevented glioblastoma growth both in vitro and in vivo. In addition, plasmaactivated medium/liquid can be prepared in advance and stored until use for cancer therapy. In this work, we designed a plasma device submerged in DI water using He, Ar, and N2 as feeding gas (Fig. 1a). The voltage and current of plasma-activated DI water were measured with a Tektronix TDS 2024B Oscilloscope. The spectra of plasma-activated DI water were characterized by UV-visible-NIR Optical Emission Spectroscopy. The temperature of the plasma solution was measured with FLIR Systems Thermal Imaging. The concentrations of ROS and RNS in DI water were determined by using a Fluorimetric Hydrogen Peroxide Assay Kit (Sigma-Aldrich, MO), and the Griess Reagent System (Promega, WI). The cell viability of the human gastric cancer cell line