Cold Stress Stimulates Intracellular Calcification by the Coccolithophore, Emiliania huxleyi (Haptophyceae) Under Phosphate-Deficient Conditions

@article{Satoh2008ColdSS,
  title={Cold Stress Stimulates Intracellular Calcification by the Coccolithophore, Emiliania huxleyi (Haptophyceae) Under Phosphate-Deficient Conditions},
  author={Manami Satoh and Koji Iwamoto and Iwane Suzuki and Yoshihiro Shiraiwa},
  journal={Marine Biotechnology},
  year={2008},
  volume={11},
  pages={327-333}
}
Intracellular calcification by the coccolith-producing haptophyte Emiliania huxleyi (NIES 837) is regulated by various environmental factors. This study focused on the relationship between cold and phosphate-deficient stresses to elucidate how those factors control coccolith production. 45Ca incorporation into coccoliths was more than 97% of the total 45Ca incorporation by whole cells. In a batch culture, orthophosphate in the medium (final concentration, 28.7 μM) was rapidly depleted within 3… 
Physiological regulation of coccolith polysaccharide production by phosphate availability in the coccolithophorid Emiliania huxleyi.
TLDR
Calcification increased gradually, but photosynthetic 14CO2 fixation was reduced by almost 40% for 5 d of culture during P(i) depletion, and production of neutral polysaccharides (NP) increased, concomitant with stimulation of cell growth.
Phosphorus limitation and heat stress decrease calcification in Emiliania huxleyi
TLDR
The predicted rise in global temperature and resulting decrease in nutrient availability may decrease CO2 sequestration by E. huxleyi through lower overall carbon production and the export of carbon may be diminished by a decrease in calcification and a weaker coccolith ballasting effect.
Association of Phosphatidylinositol-Specific Phospholipase C with Calcium-Induced Biomineralization in the Coccolithophore Emiliania huxleyi
TLDR
Findings imply that PI-PLC plays an important role in the biomineralization process of the coccolithophore E. huxleyi, and the expression of differentially expressed genes (DEGs) are investigated to observe the cellular events induced by changes in calcium concentrations.
Difference in physiological responses of growth, photosynthesis and calcification of the coccolithophore Emiliania huxleyi to acidification by acid and CO2 enrichment
TLDR
It is clearly showed that the coccolithophore, E. huxleyi, has an ability to respond positively to acidification with CO2 enrichment, but not just acidification.
Compensatory response of the unicellular-calcifying alga Emiliania huxleyi (Coccolithophoridales, Haptophyta) to ocean acidification
TLDR
The results suggest that the E. huxleyi cells possess a buffering ability to reduce acidification effects when photosynthesis is actively driven, and may not be severely damaged by oceanic acidification during photosynthesis because of their homeostatic function.
Effects of Ca and Mg on Growth and Calcification of the Coccolithophorid Pleurochrysis haptonemofera: Ca Requirement for Cell Division in Coccolith-Bearing Cells and for Normal Coccolith Formation with Acidic Polysaccharides
TLDR
It is suggested that sufficient calcification is required for the division of C-cells, under low Ca2+ and high Mg2+ conditions, and coccoliths with an abnormal morphology, having immature shield elements, were synthesized.
Phosphorus and nitrogen starvation reveal life‐cycle specific responses in the metabolome of Emiliania huxleyi (Haptophyta)
TLDR
The first comprehensive metabolome study analyzing the effect of phosphorus (P) and nitrogen (N) starvation on the diploid and haploid life-cycle stage, applying various metabolome analysis methods to gain new insights in intracellular mechanisms to cope with nutrient starvation suggests that P-starvation primarily arrests cell-cycling but that enzymatic functionality is widely preserved.
Environmental controls on coccolithophore calcification
TLDR
Variation in results of CO2 manipulation experiments can be tentatively attributed to variation among genotypes rather than differences in experimental procedure, and comparisons of methods using the same genotype, and of several genotypes using a single method, suggest significant variation amonggenotypes.
Environmental carbonate chemistry selects for phenotype of recently isolated strains of Emiliania huxleyi
TLDR
The environmental selection revealed amongst the recently isolated strain collection points to the future outcompetition of the slow growing morphotypes B/C and R by more rapidly photosynthesising, and lightly calcified strains of morphotype A but with their rate of calcification highly dependent on the surface ocean saturation state.
Changes in calcification of coccoliths under stable atmospheric CO2
The response of coccolithophore calcification to ocean acidification has been studied in culture experiments as well as in present and past oceans. The response, however, is different between species
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Results clearly showed that low temperature suppressed coccolithophorid growth but induced cell enlargement and as stimulated the intracellular calcification that produces coccoliths.
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TLDR
The isolation, cloning, and initial characterization of the first AP (ehap1) in the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler are reported, and the expression of ehap1 was found to correlate well with cellular AP activity.
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TLDR
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TLDR
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TLDR
Formation of multiple layers of coccoliths may help the cells reach deeper, nutrient‐rich water by increasing the sinking rate of the cells.
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TLDR
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TLDR
Calcite carbon and organic carbon content of the cells as well as instantaneous growth rate, cell size, chlorophyll fluorescence, and light‐scatter properties clearly depended on growth conditions and differed considerably for the two morphotypes of Emiliania huxleyi.
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