Cointegrate formation mediated by Tn9. II. Activity of IS1 is modulated by external DNA sequences.

@article{Chandler1983CointegrateFM,
  title={Cointegrate formation mediated by Tn9. II. Activity of IS1 is modulated by external DNA sequences.},
  author={Michael Chandler and David J. Galas},
  journal={Journal of molecular biology},
  year={1983},
  volume={170 1},
  pages={61-91}
}
We have studied the effect on the transposition activity of IS1 of the position and orientation of the element in the donor replicon. Previous studies have shown that the IS1s in the compound transposon Tn9 have different activities in forming plasmid cointegrates. In this study, designed to investigate the sources of this differential activity, we have cloned Tn9 (together with 132 base-pairs of flanking DNA) into several sites in pBR322 and created various derivatives of these plasmids to be… CONTINUE READING

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