Cofactor residues lysine 165 and 166 are critical for protein substrate recognition by the tissue factor-factor VIIa protease complex.

@article{Ruf1992CofactorRL,
  title={Cofactor residues lysine 165 and 166 are critical for protein substrate recognition by the tissue factor-factor VIIa protease complex.},
  author={Wolfram Ruf and David John Miles and Alnawaz Rehemtulla and Thomas Scott Edgington},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 9},
  pages={
          6375-81
        }
}
High affinity binding of factor VIIa (VIIa) to its cellular receptor tissue factor (TF), as well as association of factor X with phospholipid are required for optimal assembly of the extrinsic activation complex. In addition to the interactions of substrate with phospholipid and enzyme, we here provide evidence that cofactor residues Lys-165 and Lys-166 specifically contribute to the recognition of macromolecular substrate. Ala for Lys replacement in TFA165A166 was compatible with high affinity… CONTINUE READING

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