Coexpression of fungal phytase and xylanase utilizing the cis-acting hydrolase element in Pichia pastoris.

@article{Roongsawang2010CoexpressionOF,
  title={Coexpression of fungal phytase and xylanase utilizing the cis-acting hydrolase element in Pichia pastoris.},
  author={Niran Roongsawang and Peerada Promdonkoy and Minradee Wongwanichpokhin and Warasirin Sornlake and Aekkachai Puseenam and Lily Eurwilaichitr and Sutipa Tanapongpipat},
  journal={FEMS yeast research},
  year={2010},
  volume={10 7},
  pages={909-16}
}
Plant-based animal feed contains antinutritive agents, necessitating the addition of digestive enzymes in commercial feeds. Enzyme additives are costly because they are currently produced separately from recombinant sources. The coexpression of digestive enzymes in a single recombinant cell system would thus be advantageous. A coexpression system for the extracellular production of phytase and xylanase was established in Pichia pastoris yeast. The genes for each enzyme were fused in-frame with… CONTINUE READING