# Coexpression of a mutant β-actin and the two normal β- and γ-cytoplasmic actins in a stably transformed human cell line

@article{Vandekerckhove1980CoexpressionOA,
title={Coexpression of a mutant $\beta$-actin and the two normal $\beta$- and $\gamma$-cytoplasmic actins in a stably transformed human cell line},
author={Joël Vandekerckhove and John Leavitt and Takeo Kakunaga and Klaus Weber},
journal={Cell},
year={1980},
volume={22},
pages={893-899}
}
Abstract HUT 14 is a cloned transformed cell line derived from normal diploid human KD fibroblasts. HUT 14 cells have an altered actin phenotype. In addition to the two nonmuscle actins β and γ, also present in the parent KD cells, they show the stable expression of a novel actin species (A x -actin). Amino acid sequence analysis has been used to identify the three actins of HUT 14 cells. β- and γ-actins are identified as normal mammalian nonmuscle actins whereas A x -actin is characterized as… Expand
78 Citations
Variations in expression of mutant β actin accompanying incremental increases in human fibroblast tumorigenicity
It appears that the mutant actin of HUT-14 acquired a second-site mutation that was selected during a subcloning step prior to derivation of the H UT-14T substrain, supported by the finding that the new β-actin species in HUT -14T cells is translated correctly from HUT,14T mRNA in vitro. Expand
Expression of mutated actin gene associated with malignant transformation.
• Biology, Medicine
• Progress in nucleic acid research and molecular biology
• 1983
The expression of a mutated β-actin gene in malignant cell lines suggests the possible involvement of abnormal actin-gene expression in the neoplastic transformation process. Expand
Smooth muscle α-actin is a transformation-sensitive marker for mouse NIH 3T3 and Rat-2 cells
• Biology
• Nature
• 1985
It is shown that in NIH 3T3 and Rat-2 fibroblasts a third actin, the smooth muscle α isoform, is abundantly co-expressed with β-and γ-actin, which appears to be a reproducible transformation-sensitive marker in rodent fibro Blasts. Expand
Elevated gelsolin and α-actin expression in a flat revertant R1 of Ha-ras oncogene-transformed NIH3T3 cells
High gelsolin expression and restoration of α-actin expression may be associated with the acquirement of flat morphology and ordered cell growth pattern, which imply loss of tumorigenicity of R1 cells. Expand
Macromolecular changes accompanying immortalization and tumorigenic conversion in a human fibroblast model system.
• Biology, Medicine
• Mutation research
• 1988
Mutagenesis of a diploid human fibroblast strain, KD, with the chemical carcinogen 4 nitroquinolin-1-oxide led to the isolation of stably immortalized neoplastic substrains, which have been characterized in great detail with regard to morphology and changes in gene expression from the parental KD strain. Expand
Coexistence of three major isoactins in a single sarcoma 180 cell
• Medicine, Biology
• Cell
• 1981
Two-dimensional gel electrophoresis of the 35S-methionine-labeled polypeptides synthesized by a single sarcoma 180 cell showed that all three major actin species coexist within the same cell, and determined the ratio of gamma plus beta to zeta actin in different cytoskeletal preparations. Expand
A variant form of β-actin in a mutant of KB cells resistant to cytochalasin B
• Biology
• Cell
• 1984
Results suggest that the primary site of action of cytochalasin B on cellular motility processes is beta-actin, which is further confirmed by one-dimensional peptide analysis. Expand
Comparison of major cytoskeletons among normal human fibroblasts, immortal human fibroblasts transformed by exposure to Co-60 gamma rays, and the latter cells made tumorigenic by treatment with harvey murine sarcoma virus
• Biology, Medicine
• Experimental Gerontology
• 1987
The present data show that the morphological changes seen in the immortally transformed cells are due to the disorganization and the decrease in number of actin fibers, which seem to indicate that the immortal cells have already acquired some cancer characteristics. Expand
A developmental study of the abnormal expression of α-cardiac and α-skeletal actins in the striated muscle of a mutant mouse
• Biology
• 1989
Abstract BALB c mice possess a 5′ duplication of the α-cardiac actin gene which is associated with abnormal levels of α-cardiac and α-skeletal actin mRNAs in adult cardiac tissue. This mutationExpand
Expression of neoplasia-related proteins of chemically transformed HuT fibroblasts in human osteosarcoma HOS fibroblasts and modulation of actin expression upon elevation of tumorigenic potential.
• Biology, Medicine
• Cancer research
• 1985
It is predicted that one of two functional beta-actin genes expressed in HOS cells has been inactivated in MNNG-HOS cells by either a regulatory or structural gene mutation. Expand

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