Coenzyme a-synthesizing protein complex of Saccharomyces cerevisiae

  title={Coenzyme a-synthesizing protein complex of Saccharomyces cerevisiae},
  author={Edsel T. Bucovaz and Stanley J. Tarnowski and William C. Morrison and Robert M. Macleod and J. Calvin Morrison and Camellia M. Sobhy and James L. Rhoades and James E. Fryer and Jubran M. Wakim and Walter D. Whybrew},
  journal={Molecular and Cellular Biochemistry},
The coenzym A-synthesizing protein complex (CoA-SPC) is a multienzyme complex of Saccharomyces cerevisiae (Bakers' yeast), which has a molecular weight in excess of 200,000 as determined by Sephadex G-200 column chromatography. This multienzyme complex, which is insoluble in the crude yeast cell lysate, has been purified 229-fold. A cellular component of the yeast cell lysate, referred to as t-Factor, with a molecular weight of 400–1000 and chloride ion are involved in the solubilization of CoA… CONTINUE READING