Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells.

@article{Zhi2010CodonOO,
  title={Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells.},
  author={Ning Zhi and Zhihong Wan and Xiaohong Liu and Susan Wong and Dong Joo Kim and Neal S. Young and Sachiko Kajigaya},
  journal={Journal of virology},
  year={2010},
  volume={84 24},
  pages={13059-62}
}
Parvovirus B19 (B19V) is pathogenic for humans and has an extreme tropism for human erythroid progenitors. We report cell type-specific expression of the B19V capsid genes (VP1 and VP2) and greatly increased B19V capsid protein production in nonpermissive cells by codon optimization. Codon usage limitation, rather than promoter type and the 3' untranslated… CONTINUE READING