Codon optimization increases human kallistatin expression in Escherichia coli.

@article{Dai2013CodonOI,
  title={Codon optimization increases human kallistatin expression in Escherichia coli.},
  author={Zhiyu Dai and Yifei Chen and Weiwei Qi and Lijun Huang and Yang Zhang and Ti Zhou and Xia Yang and Guoquan Gao},
  journal={Preparative biochemistry & biotechnology},
  year={2013},
  volume={43 1},
  pages={123-36}
}
A unique serpin, kallistatin, displays vasodilatory, antiangiogenic, anti-inflammatory, and antioxidant activity. Difficulty and low efficacy of obtaining recombinant kallistatin limit the wide investigation of its biological and pathological function. The present study employed a codon optimization algorithm to redesign the kallistatin gene and achieved a high yield of recombinant kallistatin protein. The kallistatin codons were redesigned for a more suitable Escherichia coli host without… CONTINUE READING