Codon optimization and ubiquitin conjugation of human immunodeficiency virus-1 Tat lead to enhanced cell-mediated immune responses.

@article{Ramakrishna2004CodonOA,
  title={Codon optimization and ubiquitin conjugation of human immunodeficiency virus-1 Tat lead to enhanced cell-mediated immune responses.},
  author={Lakshmi Ramakrishna and Krishnamurthy Kumar Anand and Marthandan Mahalingam and Kumarasamypet M. Mohankumar and Shilpa Ramani and Nagadenahalli Byrareddy Siddappa and Udaykumar Ranga},
  journal={Vaccine},
  year={2004},
  volume={22 20},
  pages={2586-98}
}
The transactivator protein, Tat, is a potential candidate for developing a vaccine against human immunodeficiency virus (HIV-1). Since Tat is not immunodominant, especially when delivered as a genetic vaccine, we expressed codon-optimized subtype-C Tat as a molecular conjugate of ubiquitin, to elicit antigen-specific cell-mediated immune responses. Immunization of mice with different ubiquitin-Tat constructs elicited a strong cellular, but not a humoral, immune response. The combination of… CONTINUE READING

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