Coding properties of a unique apurinic/apyrimidinic site replicated in mammalian cells.

@article{Neto1994CodingPO,
  title={Coding properties of a unique apurinic/apyrimidinic site replicated in mammalian cells.},
  author={J B Cabral Neto and Rosa Estela Caseira Cabral and A. Margot and Fabien Le Page and Alain Sarasin and Aur{\'e}lien Gentil},
  journal={Journal of molecular biology},
  year={1994},
  volume={240 5},
  pages={
          416-20
        }
}
The coding properties of abasic sites have been studied in mammalian cells using a single-stranded shuttle vector carrying a unique abasic site. The probe was produced by digestion with the uracil DNA glycosylase of a uracil-containing oligonucleotide which was inserted in the single-stranded vector. After replication in monkey COS7 cells able to support DNA replication of the vector, the plasmid progeny were isolated in bacteria. DNA sequencing of rescued plasmids showed that replication of… CONTINUE READING
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