Codeine-binding RNA aptamers and rapid determination of their binding constants using a direct coupling surface plasmon resonance assay

@article{Win2006CodeinebindingRA,
  title={Codeine-binding RNA aptamers and rapid determination of their binding constants using a direct coupling surface plasmon resonance assay},
  author={Maung Nyan Win and Joshua S. Klein and Christina D. Smolke},
  journal={Nucleic Acids Research},
  year={2006},
  volume={34},
  pages={5670 - 5682}
}
RNA aptamers that bind the opium alkaloid codeine were generated using an iterative in vitro selection process. The binding properties of these aptamers, including equilibrium and kinetic rate constants, were determined through a rapid, high-throughput approach using surface plasmon resonance (SPR) analysis to measure real-time binding. The approach involves direct coupling of the target small molecule onto a sensor chip without utilization of a carrier protein. Two highest binding aptamer… CONTINUE READING