Cocktail approach for in vivo phenotyping of 5 major CYP450 isoenzymes: development of an effective sampling, extraction, and analytical procedure and pilot study with comparative genotyping.

@article{Wohlfarth2012CocktailAF,
  title={Cocktail approach for in vivo phenotyping of 5 major CYP450 isoenzymes: development of an effective sampling, extraction, and analytical procedure and pilot study with comparative genotyping.},
  author={Ariane Wohlfarth and Jana Naue and Sabine Lutz-Bonengel and Sebastian Dresen and Volker Auw{\"a}rter},
  journal={Journal of clinical pharmacology},
  year={2012},
  volume={52 8},
  pages={1200-14}
}
In this study, the authors developed a phenotyping method for CYP1A2, 2C9, 2C19, 2D6, and 3A4 using a cocktail of 100 mg caffeine, 125 mg tolbutamide, 20 mg omeprazole, 30 mg dextromethorphan, and 2 mg midazolam. A simple sampling scheme was established collecting 3 blood samples at 0, 4, and 24 hours followed by solid-phase extraction and liquid… CONTINUE READING