Cochlear gene delivery through an intact round window membrane in mouse.


Cochlear gene transfer studies in animal models have utilized mainly two delivery methods: direct injection through the round window membrane (RWM) or intracochlear infusion through a cochleostomy. However, the surgical trauma, inflammation, and hearing loss associated with these methods lead us to investigate a less invasive delivery method. Herein, we studied the feasibility of a vector transgene-soaked gelatin sponge, Gelfoam, for transgene delivery into the mouse cochlea through an intact RWM. The Gelfoam absorbed with liposomes and adenovirus, but not with adeno-associated virus (AAV), was successful in mediating transgene expression across an intact RWM in a variety of cochlear tissues. The Gelfoam technique proved to be an easy, atraumatic, and effective, but vector-dependent, method of delivering transgenes through an intact RWM. Compared with the more invasive gene delivery methods, this technique represents a safer and a more clinically viable route of cochlear gene delivery in humans.

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@article{Jero2001CochlearGD, title={Cochlear gene delivery through an intact round window membrane in mouse.}, author={Jussi Jero and A N Mhatre and Chih Jen Tseng and Rachel E. Stern and Donald E Coling and Joyce A. Goldstein and Ka Lok Hong and Wen Wen Zheng and Ariful Hoque and Anil K. Lalwani}, journal={Human gene therapy}, year={2001}, volume={12 5}, pages={539-48} }