Cocaine, not morphine, causes the generation of reactive oxygen species and activation of NF-κB in transiently cotransfected heart cells

@article{Hargrave2007CocaineNM,
  title={Cocaine, not morphine, causes the generation of reactive oxygen species and activation of NF-$\kappa$B in transiently cotransfected heart cells},
  author={Barbara Y. Hargrave and David A. Tiangco and Frank A. Lattanzio and Stephen J. Beebe},
  journal={Cardiovascular Toxicology},
  year={2007},
  volume={3},
  pages={141-151}
}
This study was designed to determine levels of NF-κB reporter gene activity and free radical generation in cultured striated myocytes (H9C2 cells) exposed to cocaine or morphine in the presence of free radical scavengers. Cells were transiently transfected with a NF-κB reporter gene and changes in luciferase activity were detected, by bioluminescence. Using confocal microscopy and 2′,7′-dichlorofluorescin diacetate, cocaine-induced or morphine-induced free radicals were quantified in H9C2 cells… 
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