Coated vesicles: characterization, selective dissociation, and reassembly.

  title={Coated vesicles: characterization, selective dissociation, and reassembly.},
  author={Melissa Woodward and Thomas F. Roth},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={75 9},
Sodium dodecyl sulfate/polyacrylamide gels of coated vesicles from porcine brain (mean 76% coated vesicles) show three major proteins (180,000, 125,000, and 55,000 daltons) that account for 73% of the total protein. Preparations consisting predominantly of coats (65%) have less of the 55,000-dalton protein. Clathrin (180,000 daltons) comprises 40% of the protein of a coated vesicle. Conditions of 2 M urea, 0.25 M MgCl2, or pH 7.5 disrupt the coat and solubilize clathrin. Solubilized clathrin… CONTINUE READING