Coated endosomal vesicles: sorting and recycling compartment for transferrin in BHK cells.

@article{Eskelinen1991CoatedEV,
  title={Coated endosomal vesicles: sorting and recycling compartment for transferrin in BHK cells.},
  author={Sinikka Eskelinen and Jan Willem Kok and Raija T. Sormunen and Dick Hoekstra},
  journal={European journal of cell biology},
  year={1991},
  volume={56 2},
  pages={210-22}
}
We have investigated receptor-mediated endocytosis of transferrin (Tf) in baby hamster kidney (BHK) cells, using fluorescence and electron microscopy, and by carrying out colocalization experiments with clathrin antibodies and a fluorescently tagged glycolipid. Early during internalization, Tf was found in small vesicles (100-150 nm in diameter) located at the cell periphery. The ligand remained associated with such vesicles when the latter concentrated towards the cell center, before ending up… CONTINUE READING