CoESPRIT: A Library-Based Construct Screening Method for Identification and Expression of Soluble Protein Complexes

@inproceedings{An2011CoESPRITAL,
  title={CoESPRIT: A Library-Based Construct Screening Method for Identification and Expression of Soluble Protein Complexes},
  author={Yingfeng An and Patrick M{\'e}resse and Philippe J. Mas and Darren J Hart},
  booktitle={PloS one},
  year={2011}
}
Structural and biophysical studies of protein complexes require multi-milligram quantities of soluble material. Subunits are often unstable when expressed separately so co-expression strategies are commonly employed since in vivo complex formation can provide stabilising effects. Defining constructs for subunit co-expression experiments is difficult if the proteins are poorly understood. Even more problematic is when subunit polypeptide chains co-fold since individually they do not have… CONTINUE READING