Co-transplantation of human hematopoietic stem cells and human breast cancer cells in NSG mice

@article{Wege2014CotransplantationOH,
  title={Co-transplantation of human hematopoietic stem cells and human breast cancer cells in NSG mice},
  author={Anja Kathrin Wege and Marcus Schmidt and Elke Ueberham and Marvin Ponnath and Olaf Ortmann and Gero Brockhoff and Jörg Lehmann},
  journal={mAbs},
  year={2014},
  volume={6},
  pages={968 - 977}
}
Humanized tumor mice (HTM) were generated by the co-transplantation of human hematopoietic stem cells and human breast cancer cells overexpressing HER2 into neonatal NOD-scid IL2Rγnull (NSG) mice. These mice are characterized by the development of a human immune system in combination with human breast cancer growth. Due to concurrent transplantation into newborn mice, transfer of MHC-mismatched tumor cells resulted in solid coexistence and immune cell activation (CD4+ T cells, natural killer… 
Human cancer evolution in the context of a human immune system in mice
TLDR
The data suggest that HIS mice may provide a promising in vivo tumor model for evaluating immune modulatory anticancer therapies, and identified different tumor models resembling specific types of human TMEs, rendering each beneficial for addressing disease‐specific issues.
Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse
TLDR
NOG-dKO mice might serve as a good humanized immunotherapy model to evaluate the efficacy of anti-PD-1 antibody prior to the clinical treatment.
Testing Cancer Immunotherapy in a Human Immune System Mouse Model: Correlating Treatment Responses to Human Chimerism, Therapeutic Variables and Immune Cell Phenotypes
TLDR
It is shown that responding tumors bear human infiltrating T cells with a more inflammatory signature compared to non-responding tumors, similar to reports of “responding” patients in human immunotherapy clinical trials.
Evaluation of anti-PD-1-based therapy against triple-negative breast cancer patient-derived xenograft tumors engrafted in humanized mouse models
TLDR
Evidence associated with anti-PD-1 immunotherapy against TNBC tumors supporting the use of TNBC PDXs in humanized mice as a model to overcome some of the technical difficulties associated with the preclinical investigation of immune-based therapies is provided.
Humanized Mice in Translational Immunology
TLDR
Significant advances have been achieved in the development of mice engrafted with functional human immune systems since the description of immunodeficient mice expressing a mutation in the IL2-receptor common gamma chain in the early 2000s and the utility of humanized mice for translating the next generation of cell-based and immunomodulatory therapies into the clinic is described.
Regulation of Programmed Death Ligand 1 (PD-L1) Expression in Breast Cancer Cell Lines In Vitro and in Immunodeficient and Humanized Tumor Mice
TLDR
It is demonstrated that the degree of PD-L1 expression amongst breast cancer cell lines varies considerably, and cytotoxic treatments and other extrinsic parameters differentially affect the expression.
Humanized Mouse Models for the Preclinical Assessment of Cancer Immunotherapy
TLDR
This review summarizes some important aspects of the different available tumor xenograft mouse models, their history, and their implementation in drug development and personalized therapy, and recent progress, opportunities and limitations of different humanized mouse models.
A novel AXL chimeric antigen receptor endows T cells with anti-tumor effects against triple negative breast cancers.
TLDR
It is shown for the first time that the receptor tyrosine kinase, AXL, is overexpressed in various tumor cell lines and patient tumor tissues, making AXL a potent novel target for cancer therapy, specifically for TNBC treatment.
Effects of Recombinant Adenovirus-Mediated Co-Transfection of CEA Gene and EPO Geneon Promoting Hematopoietic Stem Cell Directly Producing Erythrocyte Vaccine against Colon Cancer
Objective: To investigate the effects of recombinant adenovirus-mediated co-transfection of CEA gene and EPO gene on promoting hematopoietic stem cell directly producing erythrocyte vaccine against
Gr upSM Patient Derived Xenografts of Solid Tumors and Hematological Malignancies : Model Systems for Cancer Drug Development and Tu-morbiology Research
In recent years, there have been multiple efforts in the establishment and characterization of large collections of Patient Derived Tumor Xenograft (PDX) models for cancer research. Although this
...
1
2
3
...

References

SHOWING 1-10 OF 53 REFERENCES
Humanized tumor mice—A new model to study and manipulate the immune response in advanced cancer therapy
TLDR
This novel mouse model makes it possible to combine transfer of MHC mismatched tumor cells together with human HSCs resulting in a solid coexistence and interaction without evidence for rejection, and represents a powerful in vivo model that for the first time permits the investigation of human immune system‐related target cancer therapy and resistance.
Human responses against HER-2-positive cancer cells in human immune system-engrafted mice
TLDR
Human immune responses elicited in HIS mice effectively inhibited lung metastases when compared with non-vaccinated HIS mice and to non-HIS mice, along with higher levels of tumour-infiltrating human dendritic cells.
Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice
TLDR
Human cytokine gene expression by hydrodynamic delivery is a simple and efficient method to improve reconstitution of specific human-blood cell lineages in humanized mice, providing an important tool for studying human immune responses and disease progression in a small animal model.
Development of Mature and Functional Human Myeloid Subsets in Hematopoietic Stem Cell-Engrafted NOD/SCID/IL2rγKO Mice
TLDR
In vivo human myelopoiesis established in the NSG humanized mouse system may facilitate the investigation ofhuman myeloid cell biology including in vivo analyses of infectious diseases and therapeutic interventions.
GM-CSF and IL-4 Stimulate Antibody Responses in Humanized Mice by Promoting T, B, and Dendritic Cell Maturation
Engraftment of human hematopoietic stem cells into immunodeficient mice that lack T cells, B cells, and NK cells results in reconstitution of human blood lineage cells, especially B cells, in the
Development of human CD4+FoxP3+ regulatory T cells in human stem cell factor-, granulocyte-macrophage colony-stimulating factor-, and interleukin-3-expressing NOD-SCID IL2Rγ(null) humanized mice.
TLDR
In conclusion, humanized NSG-SGM3 mice might serve as a useful model to study human regulatory T-cell development in vivo, but this unexpected lineage skewing also highlights the importance of adequate spatiotemporal expression of human cytokines for future xenorecipient strain development.
NOD/SCID mice engineered to express human IL-3, GM-CSF and Steel factor constitutively mobilize engrafted human progenitors and compromise human stem cell regeneration
TLDR
It is suggested that long-term exposure of primitive human hematopoietic cells to elevated levels of human IL-3, GM-CSF and SF in vivo may deleteriously affect the stem cell compartment, while expanding terminal myelopoiesis.
Human IL-3/GM-CSF knock-in mice support human alveolar macrophage development and human immune responses in the lung
TLDR
It is demonstrated that hIL-3/GM-CSF KI mice engrafted with human CD34+ hematopoietic cells had improved human myeloid cell reconstitution in the lung and mounted correlates of a human innate immune response against influenza virus.
Antigen-specific antibody production of human B cells in NOG mice reconstituted with the human immune system.
TLDR
It is demonstrated that human T cell development is restricted by the murine MHC and consequently T cells may not achieve cognate interaction with human B cells, and suggest that if efficient cognates interaction mediated by a certain antigen on MHC class II between human T and B-2 cells occurs, human B Cells can produce IgG antibody against a given antigen in the murines environment.
Development of functional human blood and immune systems in NOD/SCID/IL2 receptor γ chainnull mice
TLDR
A new nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse line harboring a complete null mutation of the common cytokine receptor γ chain efficiently supports development of functional human hemato-lymphopoiesis.
...
1
2
3
4
5
...