Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

  title={Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers},
  author={Christian Roos and Lei Kai and Davide Proverbio and Umesh Ghoshdastider and Sławomir Filipek and Volker D{\"o}tsch and Frank Bernhard},
  journal={Molecular Membrane Biology},
  pages={75 - 89}
Abstract Routine strategies for the cell-free production of membrane proteins in the presence of detergent micelles and for their efficient co-translational solubilization have been developed. Alternatively, the expression in the presence of rationally designed lipid bilayers becomes interesting in particular for biochemical studies. The synthesized membrane proteins would be directed into a more native-like environment and cell-free expression of transporters, channels or other membrane… 
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