Co-overexpression of Escherichia coli RNA polymerase subunits allows isolation and analysis of mutant enzymes lacking lineage-specific sequence insertions.

@article{Artsimovitch2003CooverexpressionOE,
  title={Co-overexpression of Escherichia coli RNA polymerase subunits allows isolation and analysis of mutant enzymes lacking lineage-specific sequence insertions.},
  author={Irina Artsimovitch and Vladimir Svetlov and Katsuhiko S Murakami and Robert Landick},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 14},
  pages={12344-55}
}
The study of mutant enzymes can reveal important details about the fundamental mechanism and regulation of RNA polymerase, the central enzyme of gene expression. However, such studies are complicated by the multisubunit structure of RNA polymerase and by its indispensability for cell growth. Previously, mutant RNA polymerases have been produced by in vitro… CONTINUE READING