Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG.

@article{Noubir2002CoordinatedEO,
  title={Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG.},
  author={Sanaa Noubir and Ignacio Luque and Jes{\'u}s A. G. Ochoa de Alda and Ir{\`e}ne Perewoska and Nicole Tandeau de Marsac and John G Cobley and Jean Houmard},
  journal={Molecular microbiology},
  year={2002},
  volume={43 3},
  pages={749-62}
}
In the cyanobacterium Calothrix sp. PCC 7601 the cpc2 operon encoding phycocyanin 2 (PC2) is expressed if red radiations are available. RcaD was previously identified in extracts from red-light-grown cells as an alkaline phosphatase-sensitive protein that binds upstream of the transcription start point (TSP) of the cpc2 operon. In this work, RcaD was purified, and the corresponding gene cloned with a PCR probe obtained using degenerated primers based on RcaD peptide sequences (accession no… CONTINUE READING