Co-localization of the nerve growth factor precursor protein and mRNA in the mouse submandibular gland.

@article{Dicou1988ColocalizationOT,
  title={Co-localization of the nerve growth factor precursor protein and mRNA in the mouse submandibular gland.},
  author={Eleni Dicou and Junghee Lee and Philippe Brachet},
  journal={Neuroscience letters},
  year={1988},
  volume={85 1},
  pages={19-23}
}
Indirect immunofluorescence on frozen sections of the mouse submandibular gland (MSG) and affinity-purified sera directed against synthetic peptides that reproduce sequences of the nerve growth factor (NGF) precursor protein permitted its localization in the basal parts of the cells forming the secretory tubules. In situ hybridization experiments employing 35S-labeled NGF cDNA probe localized the NGF mRNA in the same region. Conversion of proNGF to mature NGF results in an altered localization… CONTINUE READING