Co-incorporation of heterologously expressed Arabidopsis cytochrome P450 and P450 reductase into soluble nanoscale lipid bilayers.

@article{Duan2004CoincorporationOH,
  title={Co-incorporation of heterologously expressed Arabidopsis cytochrome P450 and P450 reductase into soluble nanoscale lipid bilayers.},
  author={Hui Ling Duan and Natanya R Civjan and Stephen G Sligar and Mary A. Schuler},
  journal={Archives of biochemistry and biophysics},
  year={2004},
  volume={424 2},
  pages={141-53}
}
Heterologous expression of CYP73A5, an Arabidopsis cytochrome P450 monooxygenase, in baculovirus-infected insect cells yields correctly configured P450 detectable by reduced CO spectral analysis in microsomes and cell lysates. Co-expression of a housefly NADPH P450 reductase substantially increases the ability of this P450 to hydroxylate trans-cinnamic acid, its natural phenylpropanoid substrate. For development of high-throughput P450 substrate profiling procedures, membrane proteins derived… CONTINUE READING

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