Co-immobilization of semaphorin3A and nerve growth factor to guide and pattern axons.

@article{McCormick2015CoimmobilizationOS,
  title={Co-immobilization of semaphorin3A and nerve growth factor to guide and pattern axons.},
  author={Aleesha M. McCormick and Natalie A Jarmusik and Nic D. Leipzig},
  journal={Acta biomaterialia},
  year={2015},
  volume={28},
  pages={
          33-44
        }
}

A directional 3D neurite outgrowth model for studying motor axon biology and disease

A 3D motor neuron model with segregated and directed axonal outgrowth that was shown to respond to the chemotherapeutic drug vincristine in a highly reproducible dose-dependent manner and will prove ideal for studying axonal biology and disease, drug discovery and regenerative medicine.

A Hydrogel Platform that Incorporates Laminin Isoforms for Efficient Presentation of Growth Factors – Neural Growth and Osteogenesis

A new and completely defined hydrogel platform based on hybrid LM‐poly(ethylene glycol) (PEG) hydrogels with controllable stiffness (1–25 kPa) and degradability is proposed and the potential of this platform to trigger stem cell differentiation toward osteogenic lineages and stimulate neural cells growth in 3D, is demonstrated.

A Subtle Network Mediating Axon Guidance: Intrinsic Dynamic Structure of Growth Cone, Attractive and Repulsive Molecular Cues, and the Intermediate Role of Signaling Pathways

Some possible mechanisms underlying growth cone motility, relevant molecular cues, and signaling pathways in axon guidance of previous studies are summarized and some questions regarding directions for further studies are discussed.

Covalently Immobilizing Interferon-γ Drives Filopodia Production through Specific Receptor-Ligand Interactions Independently of Canonical Downstream Signaling.

It is suggested that synthetic growth factor immobilization can influence cell morphology beyond enhancing canonical cell responses through the prolonged signaling duration or spatial patterning enabled by protein immobilization.

Engineering Neuronal Patterning and Defined Axonal Elongation In Vitro

This chapter reviews techniques that have been implemented to influence the soma placement, axonal elongation, and network formation of neuronal cultures in vitro, and a comparison of manipulatable variables across methods is included.

Resolving the Interactions between Class 3 Semaphorin Receptors in Live Cells

Analysis of interactions by PIE-FCCS provides a complementary approach to the existing structural and biochemical data that will aid in the development of new therapeutic strategies to target these receptors during disease.

A Hydrogel Bridge Incorporating Immobilized Growth Factors and Neural Stem/Progenitor Cells to Treat Spinal Cord Injury

Retrograde tracing demonstrates improved neuronal regeneration through the use of immobilized growth factors in SCI, with a significant reduction in lesion area and macrophage infiltration around the lesion site.

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a

References

SHOWING 1-10 OF 58 REFERENCES

Sensory axon guidance with semaphorin 6A and nerve growth factor in a biomimetic choice point model.

The in vitro model described herein represents a versatile and valuable investigative tool in the quest for understanding developmental processes and improving regeneration following nervous system injury.

Immobilized concentration gradients of nerve growth factor guide neurite outgrowth.

The facile, flexible, and reproducible nature of this method allowed us to translate soluble growth factor gradient models to stable growth factors gradient devices that may ultimately enhance axonal guidance and regeneration in vivo.

Immobilized concentration gradients of neurotrophic factors guide neurite outgrowth of primary neurons in macroporous scaffolds.

These gradient scaffolds may be useful for guided regeneration following injury to the spinal cord or peripheral nerve and may also elucidate the mechanism for intracellular signaling of neurotrophic factors.

Light-reactive dextran gels with immobilized guidance cues for directed neurite growth in 3D models.

A novel light-reactive dextran gel for immobilizing guidance cues in neural growth models and establishes a model for investigating growth cone responses to immobilized cues in three dimensions.

Spatially patterned gene expression for guided neurite extension

A well‐defined spatially patterned gene expression system is presented to investigate neurite guidance in vitro and provides a versatile tool to investigate the factors that may promote neurite outgrowth.

Specific immobilization of biotinylated fusion proteins NGF and Sema3A utilizing a photo-cross-linkable diazirine compound for controlling neurite extension.

The results demonstrate the superiority of the immobilization approach to protein adsorption because biotinylated-fusion proteins maintain their active confirmation and their tethering can be spatially controlled via a UV activated N-MCEP-diazirine cross-linker.

Multi-Molecular Gradients of Permissive and Inhibitory Cues Direct Neurite Outgrowth

Evaluated how neurites of dorsal root ganglia neurons responded to permissive and inhibitory cues presented by substrate-bound molecular gradients, finding that neurites responded differently depending on the directions of the gradients.

Delivery of chemotropic proteins and improvement of dopaminergic neuron outgrowth through a thixotropic hybrid nano-gel

PEG-Si gel forms a functional gradient of semaphorin that enhances axon outgrowth of dopaminergic neurons from rat embryos or differentiated from stem cells in culture, and its properties allowed its injection into the striatum without inflammatory response in the short term.
...