Co-expression of the voltage-gated potassium channel Kv1.4 with transient receptor potential channels (TRPV1 and TRPV2) and the cannabinoid receptor CB1 in rat dorsal root ganglion neurons

  title={Co-expression of the voltage-gated potassium channel Kv1.4 with transient receptor potential channels (TRPV1 and TRPV2) and the cannabinoid receptor CB1 in rat dorsal root ganglion neurons},
  author={Uta Binzen and Wolfgang Greffrath and S. Hennessy and Melanie Bausen and Sigrid Saaler-Reinhardt and RD. Treede},
Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
Investigating the molecular basis of the IA in acutely dissociated DRG neurons from 7- to 8-day-old rats revealed a robust tetraethylammonium-resistant and 4-aminopyridine-sensitive IA, and possible functional roles of these channels in primary sensory neurons are discussed.
The endogenous cannabinoid anandamide inhibits transient receptor potential vanilloid type 1 receptor-mediated currents in rat cultured primary sensory neurons.
The findings suggest that activation of the CB1 receptor can reduce the activity of TRPV1 in primary sensory neurons, and that this inhibitory effect could be mediated through the reduction of PKA-mediated phosphorylation of TRpV1.
Redox modulation of A-type K+ currents in pain-sensing dorsal root ganglion neurons.
  • Chi-Pan Hsieh
  • Biology
    Biochemical and biophysical research communications
  • 2008
Ca2+/calmodulin regulates Kvβ1.1-mediated inactivation of voltage-gated K+ channels
A-type K+ channels open on membrane depolarization and undergo subsequent rapid inactivation such that they are ideally suited for fine-tuning the electrical signaling in neurons and muscle cells, and the Ca2+ dependence is gained by binding of calmodulin to the “chain” segment of Kvβ1.1 thereby compromising the mobility of the inactivation particle.
Effects of anandamide and noxious heat on intracellular calcium concentration in nociceptive drg neurons of rats.
Although anandamide acts on both TRPV1 and CB1 receptors in the same nociceptive DRG neurons, its pronciceptive effects dominate and suggest a primarily modulatory mode of action of an andamide.


Distinct potassium channels on pain-sensing neurons
It is found nerve injury induced by the Chung model of neuropathic pain leads to striking reductions in voltage-gated K+ (Kv) channel subunit expression in dorsal root ganglia (DRG) neurons, suggesting a potential molecular mechanism for hyperexcitability of injured nerves.
Co-expression of heat sensitive vanilloid receptor subtypes in rat dorsal root ganglion neurons
Rectangular temperature pulses revealed two patterns of heat-evoked inward currents in small DRG neurons: low-th threshold rapidly activating and high-threshold slowly activating.
A-type voltage-gated K+ currents influence firing properties of isolectin B4-positive but not isolectin B4-negative primary sensory neurons.
This study provides new information that 4-AP-sensitive A-type Kv currents are mainly present in IB4-positive DRG neurons and preferentially dampen the initiation of action potentials of this subpopulation of nociceptors.
Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1
It is shown that Ser229 found within the highly conserved T1 domain of Kv1.4S229.4 in cultured rat cortical neurones is phosphorylated by protein kinase A (PKA), and it is possible that neuronal transmission regulates neuronal excitability partially through the phosphorylation of S229.
Single voltage‐gated K+ channels and their functions in small dorsal root ganglion neurones of rat.
It was found that K+ conductance in small DRG neurones is formed by one type of fast inactivating A‐channel and four types of delayed rectifier K+ channels, which could be separated on the basis of their single‐channel conductance, kinetics and sensitivity to external tetraethylammonium (TEA).
Presynaptic Localization of Kv1.4-Containing A-Type Potassium Channels Near Excitatory Synapses in the Hippocampus
Mammalian Shaker voltage-gated potassium channels that contain the Kv1.4 subunit exhibit rapid activation and prominent inactivation processes, which enable these channels to integrate brief
Variation in serotonergic inhibition of calcium channel currents in four types of rat sensory neurons differentiated by membrane properties.
Rat dorsal root ganglion (DRG) cell bodies were screened according to action potential (AP) duration, capsaicin sensitivity, expression of IH, IA, and N-, L-, and T-type Ca2+ channel currents, and there was a large variation in the inhibition of high-threshold Ca2- channel currents by serotonin (5-HT) and (+)8-OH-DPAT in type 1 DRG cells versus types 2-4.
Localization and Mechanisms of Action of Cannabinoid Receptors at the Glutamatergic Synapses of the Mouse Nucleus Accumbens
Electrophysiological techniques and immunohistochemistry suggest that CB1 receptors are located on the cortical afferents to the nucleus and can reduce glutamate synaptic transmission within the NAc by modulating K+ channels activity.