Co-expression of VpROMT gene from Chinese wild Vitis pseudoreticulata with VpSTS in tobacco plants and its effects on the accumulation of pterostilbene

  title={Co-expression of VpROMT gene from Chinese wild Vitis pseudoreticulata with VpSTS in tobacco plants and its effects on the accumulation of pterostilbene},
  author={Y E Xu and Tengfei Xu and X. C. Zhao and Ying Ning Zou and Zhong Li and Jiang Xiang and F. J. Li and Y. J. Wang},
Plant secondary metabolites, such as stilbenes, have fungicidal potential and have been found in several plant species. Stilbenes in grapevine, such as resveratrol and pterostilbene, have recently attracted much attention, they are not only helping the plant to fight against pathogen attack, but they are also being widely used as ingredients of fungicide, anti-inflammatory drugs, antioxidant, and anti-infective agents. However, resveratrol O-methyltransferase gene, related with the synthesis of… Expand
O-Methyltransferases involved in biphenyl and dibenzofuran biosynthesis.
A revised biosynthetic pathway of biphenyls and dibenzofurans in the Malinae is presented and the substrate specificities of the OMTs and the regiospecificities of their reactions were rationalized by homology modeling and substrate docking. Expand
Resveratrol and Its Derivatives as Phytoalexins
Phytoalexins are plant metabolites produced in response to biotic and abiotic stressors. Stilbenes are a class of phytoalexins produced in particularly high levels in the grapevine Vitis viniferaExpand
Classification of major secondary metabolite groups is described. A short account is also given to ultraviolet (UV) climatology and UV response in plants. Investigations regarding secondaryExpand


Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata
Analysis of a series of 5′ deletions of the VpSTS promoter in grapevine leaves indicated that the proximal 162 bp from the transcription initiation site was proved to be necessary for establishing both the constitutive and induced pattern of expression. Expand
A Stress-Inducible Resveratrol O-Methyltransferase Involved in the Biosynthesis of Pterostilbene in Grapevine1
Results showed that ROMT was able to catalyze the biosynthesis of pterostilbene from resveratrol both in vitro and in planta. Expand
A Stilbene Synthase Gene (SbSTS1) Is Involved in Host and Nonhost Defense Responses in Sorghum1
The data demonstrated that SbCHS2 encodes a typical CHS that synthesizes naringenin chalcone, which is necessary for the formation of different flavonoid metabolites, suggesting that sorghum accumulates stilbene-derived defense metabolites in addition to the well-characterized 3-deoxyanthocyanidin phytoalexins. Expand
Powdery Mildew Induces Defense-Oriented Reprogramming of the Transcriptome in a Susceptible But Not in a Resistant Grapevine1[W][OA]
Results suggested that resistance to PM in V. aestivalis was not associated with overall reprogramming of the transcriptome, and induced defense-oriented transcriptional changes inV. Expand
Agrobacterium-mediated genetic transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata
A novel stilbene synthase gene (STS), cloned from Chinese wild Vitis pseudoreticulata (W. T. Wang) and responsible for synthesis of the phytoalexin resveratrol in grapevine, was successfullyExpand
Disease resistance of Vitis spp. and the production of the stress metabolites resveratrol, ε-viniferin, α-viniferin and pterostilbene
Attempts to determine the times, local sites and concentration at which stress metabolites accumulate in relation to growth of the pathogen suggest that these stress metabolites are responsible for inhibiting Growth of the pathogens in resistant interactions. Expand
Molecular cloning and functional expression of a stress-induced multifunctional O-methyltransferase with pinosylvin methyltransferase activity from Scots pine (Pinus sylvestris L.)
Recombinant PMT represents a new SAM-dependent OMT for the methylation of stress-induced pinosylvin in Scots pine needles, and had a relatively broad substrate specificity. Expand
Flavonoid 3'-O-methyltransferase from rice: cDNA cloning, characterization and functional expression.
NMR analysis showed that ROMT-9 converted flavonoids containing the 3'-hydroxy functional group such as eriodictyol, luteolin, quercetin and taxifolin into the corresponding methoxy derivatives, suggesting that ROMt-9 is an OMT with strict specificity for the 3-hydroxyl group of flavonoid compounds. Expand
Phytoalexins from the Vitaceae: biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolism.
Particular attention has been given here to stilbene phytoalexins produced by Vitaceae, specifically, their pathway of biosynthesis (including stilBenephytoalxin gene transfer experiments to other plants and their biological activity together with fungal metabolism. Expand
Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase family.
Molecular modeling suggested that a single amino acid difference was responsible for the difference in substrate discrimination between CVOMT1 and EOMT 1, and this prediction was confirmed by site-directed mutagenesis, in which the appropriate mutants of CVOMt1 (F260S) and EomT1 (S261F) were produced that exhibited the opposite substrate preference relative to the respective native enzyme. Expand