Co-elimination of mec and spa genes in Staphylococcus aureus and the effect of agr and protein A production on bacterial adherence to cell monolayers.

@article{Poston1993CoeliminationOM,
  title={Co-elimination of mec and spa genes in Staphylococcus aureus and the effect of agr and protein A production on bacterial adherence to cell monolayers.},
  author={S M Poston and G R Glancey and Jacqueline E. C. Wyatt and Thea Hogan and T. J. Foster},
  journal={Journal of medical microbiology},
  year={1993},
  volume={39 6},
  pages={422-8}
}
Phenotypic loss of protein A production was tested in six methicillin-resistant (McR) Staphylococcus aureus (MRSA) isolates and their isogenic methicillin-sensitive (McS) variants by a radiolabelled IgG-binding assay with washed cells and by Western blotting of supernates prepared from lysed washed cells. Genomic DNA was probed for homology with the protein A gene (spa) in EcoRI digests and for homology to the methicillin resistance gene (mec) in HindIII digests. The McS variants had lost… CONTINUE READING

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Genetic and physical mapping of the chromosome of Staphylococcus aureus

  • RP Novick
  • Molecular biology of the staphylococci
  • 1990

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