Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells

@inproceedings{Miryounesi2013CocultureOM,
  title={Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells},
  author={Mohammad Miryounesi and Karim Nayernia and Mahdi Dianatpour and Fatemeh Mansouri and Mohammad H Modarressi},
  booktitle={Iranian journal of basic medical sciences},
  year={2013}
}
OBJECTIVE(S) Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells. MATERIALS AND METHODS A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence protein was produced to select differentiated mESCs. To analyze sertoli cells' effect in differentiation… CONTINUE READING
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