Co-culture based blood-brain barrier in vitro model, a tissue engineering approach using immortalized cell lines for drug transport study.

@article{Zhang2011CocultureBB,
  title={Co-culture based blood-brain barrier in vitro model, a tissue engineering approach using immortalized cell lines for drug transport study.},
  author={Zhiqi Zhang and Anthony J. McGoron and Eric Crumpler and Chen-Zhong Li},
  journal={Applied biochemistry and biotechnology},
  year={2011},
  volume={163 2},
  pages={
          278-95
        }
}
This study evaluated the feasibility of using commercially available immortalized cell lines in building an in vitro blood-brain barrier (BBB) co-culture model for preliminary drug development studies. Astrocytes-derived acellular extracellular matrix (aECM) was introduced in the co-culture model to provide a novel biomimetic basement membrane for the endothelial cells to form tight junctions. Trans-Endothelial Electrical Resistance (TEER) and solute mass transport studies quantitatively… CONTINUE READING