Co-aggregation of penicillin g acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media.

@article{Wilson2004CoaggregationOP,
  title={Co-aggregation of penicillin g acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media.},
  author={Lorena Wilson and Andr{\'e}s Illanes and Olga Abian and Benevides Costa Pessela and Roberto Fernandez-Lafuente and Jos{\'e} M Guis{\'a}n},
  journal={Biomacromolecules},
  year={2004},
  volume={5 3},
  pages={852-7}
}
A novel type of biocatalyst that combines the good properties of cross-linked enzyme aggregates (CLEAs) and hydrophilic microenvironments has been developed. Dextran sulfate- and polyethyleneimine-coated CLEAs of penicillin acylase (CLEA-GDP) were prepared by adding the polymers of different sizes before the precipitation stage of the enzyme. This study presents the development and optimization of a protocol to produce such a biocatalyst using penicillin acylase as a model. Experiments show… CONTINUE READING

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