Co-Feeding Strategy of Methanol and Sorbitol to Improve Produc tion of Anti-HER2 Monoclonal Antibody in Glycoengineered Pichia pastoris

@inproceedings{Ai2013CoFeedingSO,
  title={Co-Feeding Strategy of Methanol and Sorbitol to Improve Produc tion of Anti-HER2 Monoclonal Antibody in Glycoengineered Pichia pastoris},
  author={Gao Ai},
  year={2013}
}
Objective:In this work, a study of the fermentation technique of engineered antibodies in glycosylation engineered yeast using anti-HER2 monoclonal antibody(mAb) as model was presented.Methods:The opti mal methanol induction concentration was confirmed by flask trial. The antigen binding affinity of anti-HER2 mAb was tested with the high HER2 expression breast cancer cell line SK-BR-3. The methanol/sorbitol co-feeding in duction strategy for antibody production was carried out in a 5 L… CONTINUE READING