Clusters, factories and domains: The complex structure of S-phase comes into focus.

@article{Gillespie2010ClustersFA,
  title={Clusters, factories and domains: The complex structure of S-phase comes into focus.},
  author={Peter J. Gillespie and J Julian Blow},
  journal={Cell cycle},
  year={2010},
  volume={9 16},
  pages={3218-26}
}
During S-phase of the cell cycle, chromosomal DNA is replicated according to a complex replication timing program, with megabase-sized domains replicating at different times. DNA fibre analysis reveals that clusters of adjacent replication origins fire near-synchronously. Analysis of replicating cells by light microscopy shows that DNA synthesis occurs in discrete foci or factories. The relationship between timing domains, origin clusters and replication foci is currently unclear. Recent work… CONTINUE READING