Clusterin binds by a multivalent mechanism to the Fc and Fab regions of IgG.

Abstract

Clusterin was purified from human serum by IgG and monoclonal antibody affinity chromatography. SDS-PAGE and immunoblotting revealed no major differences between clusterin prepared in these two ways. An ELISA method for measuring the binding of clusterin to immunoglobulins was developed. Clusterin purified by IgG affinity chromatography bound to pooled… (More)

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@article{Wilson1992ClusterinBB, title={Clusterin binds by a multivalent mechanism to the Fc and Fab regions of IgG.}, author={Mike Wilson and Simon B Easterbrook-Smith}, journal={Biochimica et biophysica acta}, year={1992}, volume={1159 3}, pages={319-26} }