Cloud-Point Extraction Combined with Liquid Chromatography for the Determination of Ergosterol, a Natural Product with Diuretic Activity, in Rat Plasma, Urine, and Faeces

@inproceedings{Chen2013CloudPointEC,
  title={Cloud-Point Extraction Combined with Liquid Chromatography for the Determination of Ergosterol, a Natural Product with Diuretic Activity, in Rat Plasma, Urine, and Faeces},
  author={Dan-Qian Chen and Jun-Min An and Ya-Long Feng and Ting Tian and Xiang-Yang Qin and Ying-Yong Zhao},
  booktitle={Journal of analytical methods in chemistry},
  year={2013}
}
Ergosterol from many medicinal fungi has been demonstrated to possess a variety of pharmacological activities in vivo and in vitro. A new method based on cloud-point extraction has been developed, optimized and validated for the determination of ergosterol in rat plasma, urine and faeces by liquid chromatography. The non-ionic surfactant Triton X-114 was chosen as the extract solvent. The chromatographic separation was performed on an Inertsil ODS-3 analytical column with a mobile phase… CONTINUE READING

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