Clostridium difficile genotyping based on slpA variable region in S-layer gene sequence: an alternative to serotyping.

@article{Karjalainen2002ClostridiumDG,
  title={Clostridium difficile genotyping based on slpA variable region in S-layer gene sequence: an alternative to serotyping.},
  author={Tuomo K. Karjalainen and Nicolas Saumier and Marie-Claude Barc and Michel Delm{\'e}e and Alain Collignon},
  journal={Journal of clinical microbiology},
  year={2002},
  volume={40 7},
  pages={
          2452-8
        }
}
Recent investigations of Clostridium difficile cell wall components have revealed the presence of an S-layer encoded by the slpA gene. The aim of this study was to determine whether slpA genotyping can be used as an alternative to serotyping. The variable regions of slpA were amplified by PCR from serogroup reference strains and various clinical isolates chosen randomly. Amplified products were analyzed after restriction enzyme digestion and DNA sequencing. The sequences of the variable region… CONTINUE READING
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