Closing the lid on DNA end-to-end stacking interactions.

  title={Closing the lid on DNA end-to-end stacking interactions.},
  author={Li Li and Suzette A. Pabit and Jessica S. Lamb and Hye Yoon Park and Lois Pollack},
  journal={Applied physics letters},
  volume={92 22},
Recent experiments suggest that short DNA strands associate by end-to-end stacking. Here, we report interactions between DNAs with modified ends. DNA duplexes, 20 bp long, were capped with short T(4) loops at 2, 1 or 0 ends, and were placed in solutions containing 20 mM Mg(2+). Association was observed only in constructs with one or more uncapped ends. DNA-DNA interactions were characterized by measuring variations in small angle x-ray scattering (SAXS) curves at the lowest scattering angles… CONTINUE READING