Closing the diarrhoea diagnostic gap in Indian children by the application of molecular techniques.

@article{Ajjampur2008ClosingTD,
  title={Closing the diarrhoea diagnostic gap in Indian children by the application of molecular techniques.},
  author={Sitara Swarna Rao Ajjampur and Priya Rajendran and Sasirekha Ramani and Indrani Banerjee and Bindhu Monica and Premi Sankaran and V. Vinoth Rosario and Rajesh Arumugam and Rehena Sarkar and H. Ward and Gi Seok Kang},
  journal={Journal of medical microbiology},
  year={2008},
  volume={57 Pt 11},
  pages={
          1364-8
        }
}
A large proportion of diarrhoeal illnesses in children in developing countries are ascribed to an unknown aetiology because the only available methods, such as microscopy and culture, have low sensitivity. This study was aimed at decreasing the diagnostic gap in diarrhoeal disease by the application of molecular techniques. Faecal samples from 158 children with and 99 children without diarrhoea in a hospital in South India were tested for enteric pathogens using conventional diagnostic methods… 
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References

SHOWING 1-10 OF 28 REFERENCES
Etiology of Diarrhea in Young Children in Denmark: a Case-Control Study
TLDR
The single most important cause of diarrhea was rotavirus, which points toward the need for a childhood vaccine for this pathogen, but norov virus, adenovirus, and sapovirus were also major etiologies.
Rotavirus disease in Finnish children: use of numerical scores for clinical severity of diarrhoeal episodes.
TLDR
65 episodes of rotavirus diarrhoea, detected during a longitudinal follow-up of 336 infants from birth to 24-32 months of age, were analyzed for clinical symptoms and a 0-20 point numerical score was devised according to the distribution of clinical features in the patients.
Human caliciviruses in symptomatic and asymptomatic infections in children in Vellore, South India
TLDR
This is the first report of genotyping of circulating caliciviruses in both hospital and community in India and has increased the evidence for the role of these viruses in pediatric gastroenteritis in India.
The relationship of microbial pathogens to acute infectious diarrhoea of childhood.
TLDR
The high prevalence of pathogens in controls suggested that the population may be partially protected against a variety of enteric pathogens and that final common pathways leading to diarrhoea may be activated by changes in the microbial ecology of the gut lumen.
A millennium update on pediatric diarrheal illness in the developing world.
TLDR
The major therapeutic intervention for all individuals with diarrhea consists of fluid and electrolyte therapy and when antimicrobial therapy is appropriate, selection of a specific agent should be made based upon susceptibility patterns of the pathogen or information on local susceptibility patterns.
Quantitation of group A rotavirus by real‐time reverse‐transcription‐polymerase chain reaction: Correlation with clinical severity in children in South India
TLDR
There was a significant negative correlation between severity and the PCR cycle at which the PCR amplicons were detectable (crossing point) on the assay, indicating that children with more severe diarrhoea excrete more virus than children with less severe disease.
Investigation of diarrhoeic faecal samples for enterotoxigenic, Shiga toxin-producing and typical or atypical enteropathogenic Escherichia coli in Kashmir, India.
TLDR
The present study appears to be the first report of subtype analysis of the eaeA gene from India and also records the isolation of EPEC with the EaeA-xi gene from humans.
Rotavirus gastroenteritis among children under five years of age in Izmir, Turkey.
TLDR
Rotavirus is the most common cause of severe gastroenteritis among children under five years of age in Izmir and a new potent rotavirus vaccine, when available, will provide effective protection against severe rotav virus infection.
Detection of group A rotavirus by reverse transcriptase and polymerase chain reaction in feces from children with acute gastroenteritis
TLDR
Results indicate that Rt-PCR is a sensitive and specific assay for detection of group A rotaviruses in stool samples from cases of acute diarrhoea.
Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the English case-control Infectious Intestinal Disease Study (1993–1996)
TLDR
Polymerase chain reaction (PCR) assays were applied to DNA and cDNA generated from faecal samples from cases and controls archived during the original study for the detection of norov virus, rotavirus, sapovirus, Campylobacter spp.
...
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