Closed-system solid surface vitrification versus slow programmable freezing of mouse 2-cell embryos

@article{Vutyavanich2009ClosedsystemSS,
  title={Closed-system solid surface vitrification versus slow programmable freezing of mouse 2-cell embryos},
  author={Teraporn Vutyavanich and Opas Sreshthaputra and Waraporn Piromlertamorn and Siriporn Nunta},
  journal={Journal of Assisted Reproduction and Genetics},
  year={2009},
  volume={26},
  pages={285-290}
}
To compare closed-system solid surface vitrification with slow freezing. Mouse 2-cell embryos (n = 348) were divided into vitrification, slow freezing and non-frozen groups. For vitrification, embryos were exposed to 10% ethylene glycol (EG), 10% dimethylsulfoxide (DMSO) and 10% fetal bovine serum (FBS) in phosphate-buffered saline (PBS) for 10 min, then transferred into 17.5% EG, 17.5% DMSO, 0.25 M trehalose and 10% FBS in PBS. They were placed on hemi-straws and inserted into 0.5 ml straws… CONTINUE READING

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