Corpus ID: 237490343

Closed-loop targeted optogenetic stimulation of C. elegans populations

@inproceedings{Liu2021ClosedloopTO,
  title={Closed-loop targeted optogenetic stimulation of C. elegans populations},
  author={Mochi Liu and Sandeep Kumar and Anuj K Sharma and Andrew Michael Leifer},
  year={2021}
}
We present a high-throughput optogenetic illumination system capable of simultaneous closed-loop light delivery to specified targets in populations of moving Caenorhabditis elegans. The instrument addresses three technical challenges: it delivers targeted illumination to specified regions of the animal’s body such as its head or tail; it automatically delivers stimuli triggered upon the animal’s behavior; and it achieves high throughput by targeting many animals simultaneously. The instrument… Expand

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An optogenetic illumination system capable of real-time light delivery with high spatial resolution to specified targets in freely moving Caenorhabditis elegans is presented andoptogenetic analyses of the worm motor circuit, egg-laying circuit and mechanosensory circuits that have not been possible with previous methods are performed. Expand
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An instrument is presented to simultaneously monitor and manipulate neural activity while observing behavior in a freely moving animal, the nematode Caenorhabditis elegans, to directly observe the relationship between sensory neuron activation, interneuron dynamics and locomotion in the worm's mechanosensory circuit. Expand
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