Clonogenic cell assay for anchorage-dependent squamous carcinoma cell lines using limiting dilution.

@article{Grenman1989ClonogenicCA,
  title={Clonogenic cell assay for anchorage-dependent squamous carcinoma cell lines using limiting dilution.},
  author={Reidar Axel Grenman and D L Burk and Erkki Virolainen and Ronald N. Buick and James Church and Donald R. Schwartz and Thomas E. Carey},
  journal={International journal of cancer},
  year={1989},
  volume={44 1},
  pages={131-6}
}
Clonogenic assays under either anchorage-dependent or -independent conditions are very useful for testing the sensitivity of tumor cells to cytotoxic drugs and radiation. These assays have not been widely used with squamous-cell carcinomas (SCC) because of poor tumor-cell viability and poor cloning efficiency, especially in semi-solid media. To find a clonogenic assay suitable for use with human squamous cancers we tested SCC lines, derived in our laboratory from patients with head and neck… CONTINUE READING
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