Cloning whole bacterial genomes in yeast

Abstract

Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. Here we report the cloning of… (More)
DOI: 10.1093/nar/gkq119

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